Antimicrobial Susceptibility of Multidrug-Resistant Bacteria

Antimicrobial susceptibility testing was performed against four multidrug resistant Gram-positive bacteria: Enterococcus faecalis vanB2-C3735 [28 (link)], Enterococcus faecium vanA-C2302 [29 (link)], Staphylococcus aureus C5932 (MRSA CC398) [30 (link)], Staphylococcus epidermidis C3658 (linezo-R) [31 (link)], and 4 multiresisatnt Gram-negative bacteria: Salmonella enteritidis C4220, Escherichia coli C999 (CTX-M-15) [32 (link)] Klebsiella pneumoniae C1370 (CTX-M-15) [32 (link)], Pseudomonas aeruginosa C4660 (VIM-2) [33 (link)]; and two Gram-positive foodborne strains Listeria monocytogenes ATCC700302 and Bacillus cereus ATCC1306. The strains are part of the University of Trás-os-Montes and Alto Douro and University of La Rioja collections. All the bacterial strains were subcultured from the original culture in Brain Heart Infusion (BHI) agar (Oxoid, UK) for 24 h at 37 °C. Müller-Hinton (MH) agar (Oxoid, UK) was used for the antimicrobial susceptibility assay. All the bacterial strains were subculture from the original culture in Brain Heart Infusion (BHI) agar (Oxoid, UK) for 24 h at 37 °C. Müller-Hinton (MH) agar (Oxoid, UK) was used for the antimicrobial susceptibility assay.

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Silva V., Singh R.K., Gomes N., Soares B.G., Silva A., Falco V., Capita R., Alonso-Calleja C., Pereira J.E., Amaral J.S., Igrejas G, & Poeta P. (2020). Comparative Insight upon Chitosan Solution and Chitosan Nanoparticles Application on the Phenolic Content, Antioxidant and Antimicrobial Activities of Individual Grape Components of Sousão Variety. Antioxidants, 9(2), 178.

Publication 2020

Brain Heart Infusion (BHI) agar Müller-Hinton (MH) agar

Agar Antimicrobial Assay Bacillus cereus Bacterial Brain Enterococcus faecalis Enterococcus faecium Escherichia coli Gram negative bacteria Gram positive bacteria Heart Klebsiella pneumoniae Listeria monocytogenes Mrsa Pseudomonas aeruginosa Salmonella enteritidis Staphylococcus aureus Staphylococcus epidermidis Strains Susceptibility

Corresponding Organization : Rede de Química e Tecnologia

Other organizations : Association for the Development of Douro Viticulture, Universidad de León, Polytechnic Institute of Bragança, Universidade do Porto

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Variable analysis

independent variables

Antimicrobial agents used for susceptibility testing

dependent variables

Antimicrobial susceptibility of the bacterial strains

control variables

Bacterial strains used (Enterococcus faecalis, Enterococcus faecium, Staphylococcus aureus, Staphylococcus epidermidis, Salmonella enteritidis, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Listeria monocytogenes, Bacillus cereus)
Growth media (Brain Heart Infusion agar and Müller-Hinton agar)
Incubation conditions (24 h at 37 °C)

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