Human hnRNPU cDNA (2478 bp) and CTCF cDNA (2184 bp) were subcloned into bimolecular fluorescence complementation (BiFC) vectors pBiFC-VN173 and pBiFC-VC155 (Addgene) and co-transfected into tumor cells with Lipofectamine 2000 (Invitrogen) for 24 h. Fluorescence emission was observed under a confocal microscope, using excitation and emission wavelengths of 488 and 500 nm, respectively [14 (link)].
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