YQJPF: Multiherbal Decoction Characterization

YQJPF was provided by Nanjing University of Chinese Medicine, Nanjing Hospital Affiliated to Nanjing University of Chinese Medicine. YQJPF was prepared from nine commonly used Chinese herbal medicines (Table 1). All the herbal constituents were obtained from the Beijing Tong Ren Tang Co. Ltd. (Beijing, China). Huangqi, Taizishen, Baizhu, Chenpi, Danggui, Fulin, Huangqin, and Gancao were mixed in the ratio 30:30:30:10:15:15:3:10:10.
Then, all herbs were decocted twice and the decoction liquids were concentrated to a density of 2.86 g/ml and was stored at 4°C. For quality control, HPLC analysis was used as previously described (Yao et al., 2018 (link)). YQJPF were assessed using an Agilent 1,260 liquid chromatography system (United Kingdom). Briefly, 10 μl YQJPF solution was injected into an apparatus with an auto sampler. Chromatographic separation was implemented at a flow rate of 1 ml/min with an Agilent C18 column (4.6 mm × 250 mm, 5 μm). The separation phase was composed of 0.2% phosphoric acid (A) and acetonitrile (B). The linear concentration solution gradually increases from 2 to 72% of solvent B over the course of 45 min. The separation temperature was 40°C, with a detection wavelength of 254 nm. The results are shown in Figure 1.