TERRA was masked by an LNA gapmer mediated approach as previously described [41 (link)]. The LNA gapmer contains phosphorothioate backbone modifications indicated by “*” in the sequence below.
The position of the LNA modifications and the sequence of the LNA gapmer are designed by Qiagen. At first, U2OS cells were transfected with siNC or siRBMX siRNAs. 24 h post the siRNA transfection, cells were then transfected with LNA gapmer by using Lipo3000 Reagent (Thermo Fisher Scientific) and incubated for 24 h. TERRA or control antisense LNA gapmer includes sequences 5′-T*A*A*C*C*C*T*A*A*C*C*C*T*A*A*C-3′ or 5′-C*A*C*G*T*C*T*A*T*A* C*A*C*C*A*C-3′, respectively.
The position of the LNA modifications and the sequence of the LNA gapmer are designed by Qiagen. At first, U2OS cells were transfected with siNC or siRBMX siRNAs. 24 h post the siRNA transfection, cells were then transfected with LNA gapmer by using Lipo3000 Reagent (Thermo Fisher Scientific) and incubated for 24 h. TERRA or control antisense LNA gapmer includes sequences 5′-T*A*A*C*C*C*T*A*A*C*C*C*T*A*A*C-3′ or 5′-C*A*C*G*T*C*T*A*T*A* C*A*C*C*A*C-3′, respectively.
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