The detailed method was based on previous report [31 (link)]. The cell lysates containing same amount of protein were separated on SDS polyacrylamide gels. Membranes (Millipore, Billerica, MA, USA) were incubated with antibodies against the total and phosphor-form SAPK/JNK, p50, p65, EZH2 and DNMT1 (1:1000). The membranes were washed and incubated with a secondary antibody raised against rabbit IgG conjugated to horseradish peroxidase (Cell Signaling, Beverly, MA, USA), followed by transferring to freshly made ECL solution (Immobilon Western; Millipore, Billerica, MA, USA), and documented the signals using the Gel Imagine System (Bio-Rad, Hercules, CA, USA).
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