Western Blot Analysis of Protein Expression in BMDCs

The expression of proteins of interest in BMDCs was analyzed using western blotting as previously described 1 (link). The following primary antibodies were used: anti-TGR5 (Abcam, Cambridge, MA, USA), FXR (Abcam), Bax (Abcam), BCL-2 (Abcam), caspase3 (Abcam), cleaved-caspase3 (Abcam), LC3 (Abcam), Belin1(Abcam), P62 (Abcam), P65 (Abcam), Ikbα (Abcam), ERK1/2 (Santa Cruz), P38 (Abcam), JNK (Abcam), phospho-P65 (CST), phospho-Ikbα (Abcam), phospho-ERK1/2 (Santa Cruz), phospho-P38 (Abcam), phospho-JNK (Abcam), and β-actin (Abcam).

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Hu J., Zhang Y., Yi S., Wang C., Huang X., Pan S., Yang J., Yuan G., Tan S, & Li H. (2022). Lithocholic acid inhibits dendritic cell activation by reducing intracellular glutathione via TGR5 signaling. International Journal of Biological Sciences, 18(11), 4545-4559.

Publication 2022

anti-TGR5 Bax BCL-2 caspase3 cleaved-caspase3 ERK1/2 phospho-Ikbα phospho-ERK1/2 phospho-P38 phospho-JNK β-actin

Actin Antibodies Bcl 2 Caspase3 Erk1 Proteins

Corresponding Organization :

Other organizations : First Affiliated Hospital of Chongqing Medical University, Chongqing Medical University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Expression of proteins of interest (TGR5, FXR, Bax, BCL-2, caspase3, cleaved-caspase3, LC3, Belin1, P62, P65, Ikbα, ERK1/2, P38, JNK, phospho-P65, phospho-Ikbα, phospho-ERK1/2, phospho-P38, phospho-JNK, and β-actin)

control variables

None explicitly mentioned

controls

The expression of proteins was analyzed using western blotting as previously described in the reference provided (link to Pubmed ID 33613524).

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