Pharmacological Modulation of Proteostasis

DMSO was used as vehicle control. The proteasome inhibitor MG132 was obtained from Merck/Millipore and used at the final concentration of 20 μM for 3 h. PHD inhibitors IOX2 and FG-4592 were purchased from Selleckchem. VHL inhibitors VH032, VH298, and nonbinding epimer cisVH298 were synthesized by a group member of our laboratory (13 (link), 14 (link)). Compounds were added to cells for indicated length of time. Chloroquine (Merck) and bafilomycin A1 (Selleckchem) were added to cells for 3 h at 50 μM and 50 nM, respectively.

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Frost J., Rocha S, & Ciulli A. (2021). Von Hippel–Lindau (VHL) small-molecule inhibitor binding increases stability and intracellular levels of VHL protein. The Journal of Biological Chemistry, 297(2), 100910.

Publication 2021

MG132 FG-4592 Chloroquine bafilomycin A1

Bafilomycin a1 Cells Chloroquine Dmso Fg 4592 Inhibitors Mg132 Proteasome inhibitor Vh298

Corresponding Organization : University of Dundee

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Variable analysis

independent variables

MG132 (proteasome inhibitor) at 20 μM for 3 h
IOX2 (PHD inhibitor)
FG-4592 (PHD inhibitor)
VH032 (VHL inhibitor)
VH298 (VHL inhibitor)
Cis-VH298 (nonbinding epimer of VHL inhibitor)
Chloroquine at 50 μM for 3 h
Bafilomycin A1 at 50 nM for 3 h

dependent variables

Measured outcomes not explicitly mentioned

control variables

DMSO (vehicle control)

controls

Positive control: Not specified
Negative control: DMSO (vehicle control)

Annotations

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