Sorting and Expanding T Cell Subsets

All cell populations were sorted using a FACSAria™ III (BD Biosciences, Franklin Lakes, NJ USA), and only preparations with a purity of >95% were used for further experiments. Memory CD4+ and CD8+ T cells were sorted from peripheral blood after staining with the following antibodies: anti-CD3-PE (Biolegend, San Diego, CA, USA), anti-CD4-APC (eBiosciences, San Diego, CA, USA), anti-CD8-Pacific Blue (Biolegend), and anti-CD45RO FITC (Biolegend) as previously described (22 (link)). T cells expressing specific TRBV families were sorted from bulk phytohemagglutinin-expanded CSF T cells after staining with the corresponding TRBV-specific antibodies (Beckman Coulter, Nyon, Switzerland), expanded and cloned as previously described (22 (link)).