Artemisia Extracts Inhibit Fibroblast Growth

CRL-2097 human dermal fibroblasts were acquired from ATCC and maintained in culture in DMEM/F-12 with GlutaMAX supplement (Thermo Fisher Scientific, Waltham, MA, USA, 10565018) and 10% Fetal Clone III (Cytiva, Marlboro, MA, USA, SH30109.03) and incubated at 37 °C and 5% CO₂ in high humidity. Dried leaves of Artemisia annua L. cv. SAM (voucher MASS 317314; batch B#1.Sh6.01.15.20) and Artemisia afra cv. MAL Jacq. ex Willd. (voucher FTG181107; batch B#1RbA.10.12.20) were acquired from Atelier Temenos LLC in Homestead, FL, USA. Hot-water extracts (tea) at 10 g leaf dry weight per L were prepared according to Kane et al. [44 (link)]. The ART content in the tea was 581.69 µM, as analyzed by the method described in Martini et al. [9 (link)], and was diluted to 50 µM for the experiments. For the A. afra experiments, hot-water extracts were prepared in a similar manner.