The slides containing the sections were coded and quantification performed by an experimenter blind to the code. BrdU-positive cells were counted within the SGZ [15] (link) on a Zeiss Axioskop-2 Plus microscope. The number of BrdU-positive cells per section was determined by assessing every sixth section across the rostro-caudal extent of the hippocampus (10 sections/animal). The number of DCX- and GFP-positive cells in the dentate gyrus was quantified (four sections per animal) using a Zeiss Axioscope and Nikon Eclipse 90i fluorescence microscope, respectively. The cell counts are plotted as a percentage of the control (vehicle-treated) for each adrenergic receptor agonist and antagonist. The numbers of cells/section for all treatment conditions are shown in Table S1 .
The number of Nestin-GFP/GFAP double-positive cells was quantified by determining the percentage of GFP-positive cells that colocalized with GFAP using confocal microscopy. At least 50 GFP-positive cells from each animal (four sections per animal) were analyzed using z-plane confocal sectioning with 1 µm steps on a Zeiss LSM5 Exciter microscope.
The number of Nestin-GFP/GFAP double-positive cells was quantified by determining the percentage of GFP-positive cells that colocalized with GFAP using confocal microscopy. At least 50 GFP-positive cells from each animal (four sections per animal) were analyzed using z-plane confocal sectioning with 1 µm steps on a Zeiss LSM5 Exciter microscope.
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