Cloning and Characterization of Chrysanthemum MYB Transcription Factors

Total RNA was extracted from the ray florets of chrysanthemum seedlings using the Quick RNA Isolation Kit (Huayueyang Biotechnology Co. Ltd., Beijing, China), and first-strand cDNA was synthesized by the M-MLV Reverse Transcription Kit (Promega, Madison, WI, USA) according to the manufacturer’s protocol. All of the CmMYB sequences were obtained from the chrysanthemum RNA-Seq database, which was constructed using the ray florets of the capitulum during different developmental stages and light conditions in our previous study [26 (link)]. The open reading frames of CmMYB4, CmMYB5, CmMYB6, and CmMYB7 were acquired by PCR amplification from the cDNA with specific primers (Table 1). Amplification conditions were previously described [26 (link)].