RT-qPCR was performed using SYBR Green Pro Taq HS (Accurate Biology, Changshang, China). RT-qPCR primers were designed using TBtools. The relative expression levels were calculated using the e 2−∆∆Ct method. The RT-qPCR procedure was followed according to a previously established protocol [90 (link)]. GAPDH was used as the internal reference gene.
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