The decreases in chlorophyll concentrations are related to leaf yellowing, which is a sign of termination of vase life [27 ]. As a result, the photosynthetic pigments of the leaves were evaluated under various treatments at the maximum flower diameter stage [31 (link)]. Fresh leaf samples (0.1 g) were washed and incubated overnight at a cool temperature (4-5 °C) in 5 ml of N,N-dimethyl formamide solution. A spectrophotometer (Unico W49376 Spectrophotometer 1200, China) was used to quantify chlorophyll a, b, total chlorophyll, and carotenoids at 647, 663, and 470 nm [32 (link)] formulae were used to compute chlorophylls and carotenoids (mg/g fresh weight) as follows:
Chl. a =12.70 A663 – 2.79 A647
Chl. b =20.76 A647 – 4.62 A663
Total Chls =17.90 A647 + 8.08 A663
Carotenoids = [1000 A470 – (3.72 chl. a - 104 chl. b)]/229
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