Isolation and Genome Sequencing of Saccharopolyspora from Wheat

For microbial isolation on laboratory culture medium, wheat qu samples were suspended in sterile distilled water, and serial dilutions were plated onto Gauzeˊs Medium (Sigma) for incubation at 30 °C for between 7 to 14 days. Since members of Saccharopolyspora are predominant in wheat qu and HJFM, and were likely the core functional microorganisms in HJFM, especially Saccharopolyspora hirsute, Saccharopolyspora-like colonies^{60 (link)} were isolated, purified, and preserved in 30% w/v glycerol in water at −80 °C. 16 S rRNA gene sequencing using the 16S-27F and 16S-1492 primers was used for taxonomic identification^{61 (link)}. The isolated strains were identified as three S. hirsuta (J1 and J3), two S. rectivirgula (J4 and J5), three S. shandongensis (J6 to J8), and one S. erythraea (J9). S. hirsuta J2 was deposited in China Center for Type Culture Collection under the number CCTCC M 2020103. The whole genome of S. hirsute J2, the strain with the highest activity of amylase, glucoamylase and protease, was sequenced using Nanopore PromethION platform (Oxford Nanopore Technologies Ltd, Oxford, UK) and Illumina Navaseq PE150 (Illumina, SanDiego, CA, USA) at the Beijing Novogene Bioinformatics Technology Co., Ltd.