Immunofluorescence staining was performed as in our previous study [23 (link)]. Briefly, cells were fixed in 4% PFA, washed with PBS, permeabilized in 0.02% Triton X-100, blocked with 1% bovine serum albumin for 1 h at room temperature, and incubated with Nrf2 antibody (1 : 1000, Cell Signaling Technologies) overnight at 4°C. Cells were then incubated with Alexa Fluor®546 conjugated goat anti-rabbit IgG (H + L) (1 : 1000, Invitrogen) and counterstained with Hoechst33342 (DOJINDO). The stained cells were photographed with A1 scope microscope (Zeiss, Germany).
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