The kits utilized for this project were from Cayman Chemicals (corticosterone: kit #16063; cortisol kit #560360) and the assays were run according to the manufacturer’s recommendations. Details of the kit verification have been reported previously (Tetel et al., 2022a (link); Tetel et al., 2022b (link)). Plates were incubated with samples overnight at 4°C. Plates were developed for 90 min and were read at 405 nm (SynergyLx, Biotek).
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