The aorta root was dissected, embedded and cut into sections at 6 μm. The first section was harvested when the three aortic valve cusps became visible in the lumen of the aorta, and every seventh section was harvested on one slide (10 sections per slide). Lipids were detected using oil-red O staining as previously described.17 (link) Section images were analyzed by an Olympus BX51 imaging system (Olympus, Tokyo, Japan) and quantified with Image-Pro Plus 6.0 software. The atherosclerotic plaque was expressed as a percentage of the total area of the aorta. The protocol of plaque collagen staining was performed as described previously.18 (link) Atherosclerotic plaque collagen was stained by Sirius red and the sections were analyzed by microscopy.
Protocol detail
Aortic Valve Plaque Quantification
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization : Second Affiliated Hospital of Xi'an Jiaotong University
Other organizations : Gansu Provincial Hospital
Top 5 similar protocols
Variable analysis
independent variables
- Dissection of the aorta root
- Embedding and cutting the aorta root into sections at 6 μm
- Harvesting of sections (every seventh section on one slide, 10 sections per slide)
- Lipid detection using oil-red O staining
- Atherosclerotic plaque quantification as a percentage of the total area of the aorta
- Atherosclerotic plaque collagen staining using Sirius red
- The protocol of plaque collagen staining was performed as described previously (reference 18)
- Positive control: None mentioned
- Negative control: None mentioned
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!