Tumor-bearing
mice were each injected with 5–10 MBq of 64Cu-NOTAMSN-PEG-VEGF121 or 64Cu-NOTA-MSN-PEG via tail vein before serial
PET scans. PET scans on microPET/microCT Inveon rodent model scanner
(Siemens Medical Solutions USA, Inc.), image reconstruction and ROI
analysis of the PET data were performed using described previously
procedures.33 (link) Quantitative PET data was
presented as percentage injected dose per gram of tissue (%ID/g).
After the last time-point at 22 h postinjection (p.i.), mice were
euthanized and biodistribution studies were carried out to validate
the %ID/g values and radioactivity distribution based on PET imaging
in tumor-bearing mice. Blood, U87MG tumor, and major organs/tissues
were collected and wet-weighed. The radioactivity in the tissues was
measured using a γ-counter (PerkinElmer) and presented as %ID/g
(mean ± SD).
mice were each injected with 5–10 MBq of 64Cu-NOTAMSN-PEG-VEGF121 or 64Cu-NOTA-MSN-PEG via tail vein before serial
PET scans. PET scans on microPET/microCT Inveon rodent model scanner
(Siemens Medical Solutions USA, Inc.), image reconstruction and ROI
analysis of the PET data were performed using described previously
procedures.33 (link) Quantitative PET data was
presented as percentage injected dose per gram of tissue (%ID/g).
After the last time-point at 22 h postinjection (p.i.), mice were
euthanized and biodistribution studies were carried out to validate
the %ID/g values and radioactivity distribution based on PET imaging
in tumor-bearing mice. Blood, U87MG tumor, and major organs/tissues
were collected and wet-weighed. The radioactivity in the tissues was
measured using a γ-counter (PerkinElmer) and presented as %ID/g
(mean ± SD).
