Gene Expression Profiling of Cell Lines

Gene expression profiles of the wild-type and mutant cell lines were measured using SurePrint G3 Human Gene Expression 8 × 60 Kv2 Microarray (Agilent Technologies, Santa Clara, CA, USA) following the manufacturer’s protocol and are described in detail elsewhere ([49 (link)] and references therein). Background-corrected expression values were extracted using Agilent Feature Extraction Software. Data was log-transformed and quantile normalized using Agilent GeneSpring Software. Fold-changes for all genes were calculated as ratio of mutant vs. wild-type.

Free full text: Click here

Menegatti J., Nakel J., Stepanov Y.K., Caban K.M., Ludwig N., Nord R., Pfitzner T., Yazdani M., Vilimova M., Kehl T., Lenhof H.P., Philipp S.E., Meese E., Fröhlich T., Grässer F.A, & Hart M. (2022). Changes of Protein Expression after CRISPR/Cas9 Knockout of miRNA-142 in Cell Lines Derived from Diffuse Large B-Cell Lymphoma. Cancers, 14(20), 5031.

Publication 2022

SurePrint G3 Human Gene Expression 8 × 60 Kv2 Microarray Agilent Feature Extraction Software Agilent GeneSpring Software

Cell lines Genes Microarray

Corresponding Organization : Saarland University

Other organizations : Ludwig-Maximilians-Universität München

Top 5 similar protocols

Variable analysis

independent variables

Wild-type cell line
Mutant cell line

dependent variables

Gene expression profiles

control variables

SurePrint G3 Human Gene Expression 8 × 60 Kv2 Microarray (Agilent Technologies, Santa Clara, CA, USA)
Agilent Feature Extraction Software
Agilent GeneSpring Software

controls

No positive or negative controls were explicitly mentioned in the input information.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!