Sections of prostate tissue specimens were prepared from clinical prostatectomy specimens in the First Hospital of China Medical University. The procedure was performed as previously described (28 (link),31 (link)). Tissue sections of formalin-fixed, paraffin embedded tissue blocks were dewaxed in xylene, rehydrated in decreasing concentrations of ethanol, and blocked for endogenous peroxidase activity using 3% hydrogen peroxide. Antigen retrieval was carried out in citrate buffer (pH 6.0) at 130°C for 2 min. Then, non-specific antibody binding was blocked by incubating with goat serum for 15 min at room temperature. Slides were incubated with anti-BAP18 (1:100) overnight at 4°C. After washing, slides were incubated with biotinylated goat anti-rabbit/mouse immunoglobulin, followed by incubation with avidin DH-biotinylated horseradish peroxidase complex (UltraSensitiveTM SP(Mouse/Rabbit)IHC Kit, Maixin Bio). The sections were developed with the diaminobenzidine substrate kit (Maixin Bio) and counterstained with hematoxylin. Images were taken with an Olympus microscope. This study was approved by the Ethics Committee of the Medical Faculty of the China Medical University.
Protocol detail
Immunohistochemical Analysis of BAP18 in Prostate Tissue
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Anti immunoglobulin
Antibody
Antigen
At 130
Avidin horseradish peroxidase complex
Buffer
Citrate
Embedded paraffin
Ethanol
Ethics committee
Formalin
Goat
Hematoxylin
Hydrogen peroxide
Medical faculty
Microscope
Mouse
Peroxidase
Prostate
Prostatectomy
Rabbit
Serum
Tissue
Xylene
Corresponding Organization : China Medical University
Other organizations : First Hospital of China Medical University, Northeastern University
Top 5 similar protocols
Protocol cited in 1 other protocol
Variable analysis
independent variables
- Anti-BAP18 antibody concentration (1:100)
- BAP18 protein expression in prostate tissue specimens
- Tissue preparation (formalin-fixed, paraffin-embedded)
- Tissue processing (dewaxing, rehydration, blocking for endogenous peroxidase activity)
- Antigen retrieval (citrate buffer, 130°C, 2 min)
- Blocking of non-specific antibody binding (goat serum, 15 min)
- Incubation time and temperature for anti-BAP18 antibody (overnight, 4°C)
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!