Biofilms were formed as described above and triplicate biofilms were pooled, 50 µl serially diluted and plated on cetrimide agar or YPD supplemented with 100 µg ml−1 tetracycline to check for contamination. Remaining biofilm cells were centrifuged at 3500 r.p.m at 4 °C for 5 min and pellets snap frozen in liquid nitrogen. Four biological replicates were shipped to GeneWiz, UK, for RNA extraction sequencing and basic bioinformatic analysis.
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