The method for the measurement of uterine contraction has been previously described (15 (link)). Briefly, the uterine muscle strips are fixed to the constant temperature bath and multi-channel physiological signal acquisition and processing system while continuously ventilated with 95% O2 and 5% CO2 at 37.0 ± 0.5°C. Our previous study has confirmed that uterus strip contractility performance is most appropriate at a 2-g stretch (15 (link)). After the appearance of a stable and regular contraction curve, the strips were stimulated with 96 mM KCl and different concentrations of oxytocin (from 10-11 to 10-6 mM), and the contraction response was recorded. Finally, the uterine muscle strips were weighed at the end of the experiment for calibration.
Quantitative analysis of the contractility of the uterine muscle strips was performed by a multichannel physiological signal acquisition system (RM6240E, Chengdu, China) by calculating the area under the curve (AUC) of the contraction curve presented as AUC/g tissue weight. The AUC was measured at time 0 and was subtracted from the AUC measured after 5 min of application of KCl or each oxytocin concentration.
Quantitative analysis of the contractility of the uterine muscle strips was performed by a multichannel physiological signal acquisition system (RM6240E, Chengdu, China) by calculating the area under the curve (AUC) of the contraction curve presented as AUC/g tissue weight. The AUC was measured at time 0 and was subtracted from the AUC measured after 5 min of application of KCl or each oxytocin concentration.
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