Protocol detail

Isolation and Culture of hPD-MSCs

Find Similar Protocols

hPD-MSCs were kindly provided by Gi Jin Kim (CHA University, Pangyo, Korea). hPD-MSCs were isolated and characterized as reported previously [77 (link),78 (link)]. Briefly, hPS-MSCs were cultured in α-MEM containing 10% fetal bovine serum (FBS; Corning Inc., Corning, NY, USA), 1% penicillin-streptomycin (Life Technologies, Carlsbad, CA, USA), 1 mg/mL heparin, and 100 μg/mL FGF4 (PeproTech, Rocky Hill, NJ, USA) with 5% CO₂ at 37 °C.

Free full text: Click here

Kim K.H, & Lee K.A. (2022). Metabolic Rewiring by Human Placenta-Derived Mesenchymal Stem Cell Therapy Promotes Rejuvenation in Aged Female Rats. International Journal of Molecular Sciences, 23(1), 566.

Publication 2022

FBS penicillin-streptomycin heparin

Fgf4 Heparin Penicillin Streptomycin

Top 5 similar protocols

Variable analysis

independent variables

Cell type (hPD-MSCs)

dependent variables

Not explicitly mentioned

control variables

Culture medium (α-MEM containing 10% fetal bovine serum (FBS), 1% penicillin-streptomycin, 1 mg/mL heparin, and 100 μg/mL FGF4)
Incubation conditions (5% CO2 at 37 °C)

controls

Positive control: Not specified
Negative control: Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!