Immunohistochemical Staining Protocols

The staining methods were previously described (Zhu et al., 2007 (link), 2009 (link), 2013 (link); Peng et al., 2012 (link)). The antibodies for staining were purchased from the following sources: IL-17c antibody (mouse monoclonal, R&D Systems); IL-17RE antibody (rabbit polyclonal, Sigma-Aldrich); IL-17RA antibody (rabbit monoclonal, LifeSpanBioSciences); NCAM antibody (mouse monoclonal, BD); PGP9.5 (Abcam); NF200 antibody (rabbit polyclonal, Sigma-Aldrich); Peripherin antibody (rabbit and mouse, Sigma-Aldrich); cleaved caspase 3 antibody (rabbit polyclonal, Cell Signaling Technology).

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Peng T., Chanthaphavong R.S., Sun S., Trigilio J.A., Phasouk K., Jin L., Layton E.D., Li A.Z., Correnti C.E., De van der Schueren W., Vazquez J., O’Day D.R., Glass I.A., Knipe D.M., Wald A., Corey L, & Zhu J. (2017). Keratinocytes produce IL-17c to protect peripheral nervous systems during human HSV-2 reactivation. The Journal of Experimental Medicine, 214(8), 2315-2329.

Publication 2017

NF200 antibody cleaved caspase 3 antibody

Antibodies Antibody Caspase 3 Il 17c Il 17ra Mouse Ncam Peripherin Pgp9 5 Rabbit

Corresponding Organization : Fred Hutch Cancer Center

Other organizations : Harvard University

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Variable analysis

independent variables

Staining methods (previously described in Zhu et al., 2007, 2009, 2013; Peng et al., 2012)

dependent variables

Expression of IL-17c, IL-17RE, IL-17RA, NCAM, PGP9.5, NF200, Peripherin, and cleaved caspase 3

control variables

Not explicitly mentioned

controls

Positive controls: Not specified
Negative controls: Not specified

Annotations

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