Protein expression analysis by Western blot

Western blot was used to detect the protein content of AQP1-4, kidney injury molecule-1 (KIM-1), α-smooth muscle actin (α-SMA), and vimentin. The steps were as previously described[26 (link)]. The primary antibodies used for Western blot were purchased from Abcam (KIM-1: ab190696; α-SMA: ab32575; vimentin: ab92547; AQP1: ab168387; AQP4: ab46182). Grey bands on the picture were semi-quantitatively analyzed with ImageJ, with GAPDH used as the internal control.

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Hu J., Zhang Y.M., Miao Y.F., Zhu L., Yi X.L., Chen H., Yang X.J., Wan M.H, & Tang W.F. (2020). Effects of Yue-Bi-Tang on water metabolism in severe acute pancreatitis rats with acute lung-kidney injury. World Journal of Gastroenterology, 26(43), 6810-6821.

Publication 2020

ab32575 ab92547 ab168387 ab46182

Actin Antibodies Aqp1 Aqp1 protein Gapdh Kidney injury molecule 1 Smooth muscle Vimentin Western blot

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Variable analysis

independent variables

Protein content of AQP1-4, KIM-1, α-SMA, and vimentin

dependent variables

Grey band intensity on Western blot images analyzed with ImageJ

control variables

GAPDH used as internal control

controls

Positive control: Not specified
Negative control: Not specified

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