Western Blot Analysis for Protein Markers

Western blot analysis was conducted as previously described.23 (link) Primary antibodies, including Flotillin-1 (ab41927), Tsg101 (ab125011), CD63 (ab217345), p21 (ab188224), p16^INK4a (ab211542), and β-actin (ab179467) were purchased from Abcam. PNUTS (14171) was purchased from Cell Signaling Technology.

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Xia W., Chen H., Chen D., Ye Y., Xie C, & Hou M. (2020). PD-1 inhibitor inducing exosomal miR-34a-5p expression mediates the cross talk between cardiomyocyte and macrophage in immune checkpoint inhibitor–related cardiac dysfunction. Journal for Immunotherapy of Cancer, 8(2), e001293.

Publication 2020

β-actin (ab179467)

Actin Antibodies Flotillin 1 P16ink4a Tsg101 Western blot analysis

Corresponding Organization :

Other organizations : XinHua Hospital, Wenzhou Medical University, First Affiliated Hospital of Wenzhou Medical University, Radiation Oncology Associates

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Variable analysis

independent variables

No independent variables explicitly mentioned.

dependent variables

Flotillin-1
Tsg101
P16^INK4a
β-actin
PNUTS

control variables

No control variables explicitly mentioned.

controls

Positive control: Not specified.
Negative control: Not specified.

Annotations

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