Murine BMDMs were obtained as described17 (link) and maintained in DMEM supplemented with 10% FBS and 10% L929 cell supernatant as conditioned medium providing macrophage colony stimulating factor (M-CSF). Recombinant mouse IFN-γ was from Peprotech and used at 10 ng/ml. Cell culture grade LPS and CHX were purchased from Sigma-Aldrich. Pam3Cys was purchased from EMC Microcollections. LPS was used as 1 ng/ml and Pam3Cys was used at 10 ng/ml unless otherwise noted. A γ-secretase inhibitor GSI-34 was used at 10 µM as previously described46 (link). U0126, SB203580, SP600125, and CGP57380 were from Calbiochem.
Protocol detail
Murine Bone Marrow-Derived Macrophage Isolation and Stimulation
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization : Cornell University
Other organizations : Hospital for Special Surgery, Columbia University Irving Medical Center, Kiel University, Memorial Sloan Kettering Cancer Center, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health
Top 5 similar protocols
Protocol cited in 8 other protocols
Variable analysis
independent variables
- Recombinant mouse IFN-γ (10 ng/ml)
- LPS (1 ng/ml)
- Pam3Cys (10 ng/ml)
- γ-secretase inhibitor GSI-34 (10 µM)
- Not explicitly mentioned
- DMEM supplemented with 10% FBS and 10% L929 cell supernatant as conditioned medium providing macrophage colony stimulating factor (M-CSF)
- Cell culture grade LPS and CHX from Sigma-Aldrich
- Pam3Cys from EMC Microcollections
- U0126, SB203580, SP600125, and CGP57380 from Calbiochem
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!