ChIP-qPCR was performed using QuantiFast SYBR Green PCR kit according to the manufacturer’s instructions and as described in ref. 69 (link). Each reaction was performed in triplicates using a Roche LightCycler 480 system version 1.1.0.1320. The results were analysed with absolute quantification/2nd derivative maximum and the 2(−ΔC(t)) method as previously described70 (link). We used primers for TERs TER302, TER603 and TER1004 previously described in ref. 16 (link) and presented in Supplementary Table 2 . Statistical analyses (Student’s t-test, unpaired, two tailed) were performed with Numbers version 10.3.5 (7029.5.5) and the obtained p-values are indicated in figures. All experiments were performed with at least three biological replicates. Antibodies used: anti-FLAG (mouse monoclonal, clone M2, Sigma; cat. no: F3165), 5 μg antibody used for 2.5 × 108 cells; anti-myc (mouse monoclonal), clone 9E10, Santa Cruz Biotechnology cat. no. sc-40), 5 μg antibody used for 2.5 × 108 cells.
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