Chromatin Immunoprecipitation and qPCR Analysis

Cells were crosslinked, lysed and sonicated according to^{42 (link)}, then STAT5 And RNA polymerase II immunoprecipitation was performed according to^{43 (link)}. The antibodies used for immunoprecipitation were STAT5 A (sc-1081, Santa Cruz Biotechnology, Santa Cruz, CA, USA) and STAT5 B (sc-835, Santa Cruz Biotechnology), 1.2 µg each, and RNA Polymerase II CTD (MABI0601, Clinisciences, Nanterre, France), 2 µg. DNA from ChIP was isolated with a phenol/chloroform extraction, and used for qPCR with the primers listed in Supplementary Table 1. Results were then normalized using the percent input method.