Calf thymus whole histones and human histone H3.3 (expressed in E. coli) were purchased from Millipore-Sigma (Oakville, ON, Canada). Sequencing-grade modified trypsin and Glu-C were purchased from Promega (Madison, WI, USA). Atrazine (analytical standard grade) was purchased from Sigma-Aldrich (Oakville, ON, Canada). A concentration of 0.5 M phosphate-buffered saline (PBS, pH = 7.4) was purchased from Fisher Sci. (Ottawa, ON, Canada). HPLC-grade acetonitrile (ACN), dimethyl sulfoxide (DMSO), ammonium bicarbonate (NH4HCO3), formic acid, and a BCA protein assay kit were from Millipore-Sigma. Water was purified on a Milli-Q system (Millipore, Billerica, MA, USA). Asn-Asn-Asn peptide (Asn3) was synthesized by Biomatik Corporation (Kitchener, ON, Canada).
Atrazine spike solution was prepared by dissolving an amount of Atrazine in DMSO and stored at 4 °C until further use. Calf thymus whole-histone solution was prepared freshly on the day of study by dissolving an amount of histone in 50 mM PBS (pH = 7.4).
Atrazine spike solution was prepared by dissolving an amount of Atrazine in DMSO and stored at 4 °C until further use. Calf thymus whole-histone solution was prepared freshly on the day of study by dissolving an amount of histone in 50 mM PBS (pH = 7.4).
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