Recombinant pollen allergens from birch (Betula pendula) and timothy grass (Phleum pratense) pollen allergens Bet v 1.0101 and Phl p 5.0101, referred to as Bet v 1 and Phl p 5 hereafter, were obtained from Biomay AG (Vienna, Austria). Ovalbumin (OVA) was purchased from InvivoGen (Toulouse, France) and was treated the same way as the allergens to serve as a negative control in the cell culture experiments described below. Protein stock solutions (1 mg mL−1) for chemical modification were prepared with pure water as described in Backes et al. (24 (link)).
Reinmuth-Selzle K., Bellinghausen I., Leifke A.L., Backes A.T., Bothen N., Ziegler K., Weller M.G., Saloga J., Schuppan D., Lucas K., Pöschl U, & Fröhlich-Nowoisky J. (2023). Chemical modification by peroxynitrite enhances TLR4 activation of the grass pollen allergen Phl p 5. Frontiers in Allergy, 4, 1066392.
Corresponding Organization : Max Planck Institute for Chemistry
Other organizations :
University Medical Center of the Johannes Gutenberg University Mainz, Johannes Gutenberg University Mainz, Federal Institute For Materials Research and Testing, Harvard University, Beth Israel Deaconess Medical Center
Chemical modification of the recombinant pollen allergens Bet v 1 and Phl p 5
dependent variables
Outcomes measured in the cell culture experiments described
control variables
Ovalbumin (OVA) treated the same way as the allergens to serve as a negative control
controls
Ovalbumin (OVA) as a negative control
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