Immunoblot Analysis of Apoptosis Markers

Subconfluent MDA-MB-231 TD, MDA-MB-436 TD, BT-549 TD cells were treated with either Vinorelbine, vehicle control, or Staurosporine. All cells, any suspended and attached, were harvested, lysed with a RIPA lysis buffer, and analyzed by immunoblot as previously described [24 (link)] with the following antibodies: Anti-Poly (ADP-ribose) polymerase (PARP; 1:1000, SCBT), Anti-acetyl-α-tubulin (Lys40) (D20G3) XP Rabbit mAb (1:1000; Cell Signaling), Anti-detyrosinated- α-tubulin Monoclonal (1:10,000: RevMAb) or Anti-detyrosinated-α-tubulin (1:1000; Abcam), Monoclonal Anti-α-tubulin Clone DM1A (1:2000; Sigma Aldrich) and β-actin (1:5000; AC-15, Sigma).

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Thompson K.N., Ju J.A., Ory E.C., Pratt S.J., Lee R.M., Mathias T.J., Chang K.T., Lee C.J., Goloubeva O.G., Bailey P.C., Chakrabarti K.R., Jewell C.M., Vitolo M.I, & Martin S.S. (2022). Microtubule disruption reduces metastasis more effectively than primary tumor growth. Breast Cancer Research : BCR, 24, 13.

Publication 2022

Anti-acetyl-α-tubulin (Lys40) (D20G3) XP Rabbit mAb Anti-detyrosinated-α-tubulin Monoclonal Anti-α-tubulin Clone DM1A β-actin AC-15

Actin Antibodies Buffer Cells Clone Immunoblot Poly adp ribose polymerase Rabbit Ripa Staurosporine Vinorelbine α tubulin

Corresponding Organization :

Other organizations : University of Maryland, Baltimore, University of Maryland, College Park

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Variable analysis

independent variables

Vinorelbine
Vehicle control
Staurosporine

dependent variables

Poly (ADP-ribose) polymerase (PARP)
Acetyl-α-tubulin (Lys40)
Detyrosinated-α-tubulin
α-tubulin
β-actin

control variables

Subconfluent MDA-MB-231 TD, MDA-MB-436 TD, BT-549 TD cells

positive controls

Not explicitly mentioned

negative controls

Vehicle control

Annotations

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