BP strips with dimensions of 20 mm by 4 mm by 0.3 mm were cut from the membrane using a laser cutter. The strips were tested using a SAXS machine (SAXSpoint 2.0). The obtained diffraction patterns were analyzed by the software Fit2D. The diffraction maxima occurring at angles 2θ to the x-ray beam (Bragg reflections) are given by the equation: 2sin(θ) = Λn/d, where n is the order of the reflection, Λ is the wavelength of x-ray, and d is the spacing between the d-bands on collagen fibrils.
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SAXS Analysis of Collagen Fibrils
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Fibrils collagen
Membrane
Reflection
X ray
Corresponding Organization : Harvard University
Other organizations : Xijing Hospital, Air Force Medical University
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Variable analysis
independent variables
- Dimensions of BP strips (20 mm by 4 mm by 0.3 mm)
- Diffraction patterns obtained using the SAXS machine (SAXSpoint 2.0)
- Bragg reflections (diffraction maxima occurring at angles 2θ to the x-ray beam)
- Laser cutter used to cut the BP strips
- SAXS machine (SAXSpoint 2.0) used to obtain the diffraction patterns
- Fit2D software used to analyze the diffraction patterns
- Wavelength of x-ray (Λ)
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