Profiling Long Noncoding RNAs in Monocytes

RNA was isolated from purified monocytes with Tri Reagent (Sigma-Aldrich, Dublin, Ireland) as per the manufacturer’s instructions. The quality and the concentration of pooled RNA samples were monitored at absorbance ratios of A260/A280 and A260/A230 using a NanoDrop 8000 spectrophotometer. Expression profiling studies were performed on RNA by Arraystar, Inc. (Rockville, MD, USA). Briefly, labeled cRNAs were hybridized onto the Human LncRNA Array v3.0 (8 × 60 K, Arraystar) and after having washed the slides, the arrays were scanned by the Agilent Scanner G2505C. Approximately 30,600 lncRNAs and 26,109 coding transcripts can be detected using this microarray [11 (link)].