Mycological Diagnosis of Tinea Capitis

Diagnosis of TC was established at the LPM/ADUH in Dakar, on the basis of mycological examination including direct microscopy and culture as previously described [7 (link)]. Microscopic direct examination of all specimens was carried out in 20% KOH mount. All specimens were cultured on two plates/tubes, one containing Sabouraud dextrose agar (SDA) supplemented with chloramphenicol (Bio-Rad, Paris, France), and the other one containing SDA supplemented with chloramphenicol plus cycloheximide (Bio-Rad, France). Cultures were incubated at 25–30 °C and evaluated for growth after 48 h and then once weekly for a month. Positive specimens for dermatophytes were identified according to three criteria: growth rate, macroscopic and microscopic characteristics of colonies and sometimes on biochemical characteristics such as a urease test [8 ,9 (link)].

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Ndiaye M., Sacheli R., Diongue K., Adjetey C., Darfouf R., Seck M.C., Badiane A.S., Diallo M.A., Dieng T., Hayette M.P, & Ndiaye D. (2021). Evaluation of the Multiplex Real-Time PCR DermaGenius® Assay for the Detection of Dermatophytes in Hair Samples from Senegal. Journal of Fungi, 8(1), 11.

Publication 2021

Sabouraud dextrose agar chloramphenicol cycloheximide

Agar Chloramphenicol Cycloheximide Dermatophytes Dextrose Diagnosis Microscopic Urease

Corresponding Organization : Hôpital Aristide Le Dantec

Other organizations : University of Liège

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Variable analysis

independent variables

Mycological examination including direct microscopy and culture

dependent variables

Diagnosis of TC

control variables

Culture on two plates/tubes, one containing Sabouraud dextrose agar (SDA) supplemented with chloramphenicol, and the other containing SDA supplemented with chloramphenicol plus cycloheximide
Incubation at 25–30 °C and evaluation for growth after 48 h and then once weekly for a month

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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