Clinical samples from various illnesses in the ICU (n = 79) were prepared for culture using traditional techniques (cultures on routine media such as blood agar or MacConkey agar and selective media with specific biochemical tests when necessary) where all the clinical isolates have proceeded for culture on blood and MacConkey agars and then Gram staining was performed to identify bacterial morphology and Gram reaction. The studies were cultured several times to ensure that all isolates were pure. Several biochemical tests were performed to confirm that all isolates belonged to A. baumannii, including oxidase, catalase, and indole tests. Standard phenotypic assays were used for the initial identification [25 (link)].
Vitek 2 system (BioMerieux, Marcy-l’Étoile, France) was used in the microbiology laboratory to confirm the identification of the isolates, following the manufacturer’s instructions. All isolates were investigated for antibiotic susceptibility, using this automated Vitek 2 Compact system. The included samples were cultured on blood agars, and then the suspension was made for every single isolate. A liquid suspension of the studied isolates was loaded on the Vitek system, and left overnight to obtain the result. The next day, results illustrated the samples’ identification and antibiotic susceptibility.
VITEK 2 system was approved for authenticating the names of Acinetobacter spp. as described by the manufacturer (BioMerieux). The Vitek2 card contains 64 wells holding different fluorescent biochemical assays. Twenty out of the sixty-four wells were carbohydrate assimilation; four are phosphatase, nitrate, urea, and actidione tests. The machine controlled this card automatically, including filling, sealing, and finally transferring such cards into the linked incubator at a temperature of 35 °C. Each output report is usually decoded according to a specific algorithmic system. The acquired results were recognized and recorded. Most known Acinetobacter spp. have clear-cut profiles, and the system led to the correction of the unknown organism.
The susceptibility of the most commonly used antibiotics (n = 13) for the prevalent ICU infections has been recorded.
Vitek 2 system (BioMerieux, Marcy-l’Étoile, France) was used in the microbiology laboratory to confirm the identification of the isolates, following the manufacturer’s instructions. All isolates were investigated for antibiotic susceptibility, using this automated Vitek 2 Compact system. The included samples were cultured on blood agars, and then the suspension was made for every single isolate. A liquid suspension of the studied isolates was loaded on the Vitek system, and left overnight to obtain the result. The next day, results illustrated the samples’ identification and antibiotic susceptibility.
VITEK 2 system was approved for authenticating the names of Acinetobacter spp. as described by the manufacturer (BioMerieux). The Vitek2 card contains 64 wells holding different fluorescent biochemical assays. Twenty out of the sixty-four wells were carbohydrate assimilation; four are phosphatase, nitrate, urea, and actidione tests. The machine controlled this card automatically, including filling, sealing, and finally transferring such cards into the linked incubator at a temperature of 35 °C. Each output report is usually decoded according to a specific algorithmic system. The acquired results were recognized and recorded. Most known Acinetobacter spp. have clear-cut profiles, and the system led to the correction of the unknown organism.
The susceptibility of the most commonly used antibiotics (n = 13) for the prevalent ICU infections has been recorded.
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