Mouse Strain Characterization for Research

C57BL/6J (JAX 000664), FVB/NJ (FVB, JAX 001800), 129S1/SvImJ (SV129, JAX 002448), B6.Cg-Gt(ROSA)26Sor^{tm14(CAG-TT)Hze}/J (49 (link)) (R26R-TT, JAX 007914), and NOD.Cg-Prkdc^scid/J (NOD/SCID, JAX 001303) were purchased from the Jackson Laboratory. CD1 mice (022) were purchased from Charles River Laboratories. Flt3Cre⁺ ROSA^mTmG/mTmG mice (50 (link)–52 (link)) were a gift from Slava Epelman, University of Toronto, Toronto, Ontario, Canada. Tie2CreERT2 mice (gift of Ye Zheng, Cincinnati Children’s Hospital Medical Center, University of Cincinnati, Cincinnati, Ohio, USA), previously described (16 (link)), were crossed with the commercially available R26R-TT mice (above) to generate (Tie2TT) mice. The primers used for genotyping the abovementioned Cre or ROSA mice are shown in Supplemental Table 3. To induce Cre expression in Tie2CreERT2 and Cdh5(PAC)-CreERT2 mice, 50 mg/kg TAM was injected into the animals i.p. at appropriate time points (3–5 days).