Transient Expression of NSP1 and β-TrCP

The construction of CMV expression vectors pLIC6 and pLIC6F, used for transient expression of wild-type and mutant forms of NSP1 or FLAG-tagged β-TrCP, were described previously (5 (link)). pcDNA3-DN-hCUL3-FLAG was a gift from Wade Harper (Addgene plasmid 15820) (3 (link)). pHTN-NSP1(OSU) was kindly provided by Michelle Arnold (9 (link)). Plasmids were grown in TOP10 Escherichia coli cells (Thermo, Fisher), purified using NucleoBond Xtra endotoxin-free plasmid purification kits (Clontech), and verified by sequencing.

Free full text: Click here

Davis K.A., Morelli M, & Patton J.T. (2017). Rotavirus NSP1 Requires Casein Kinase II-Mediated Phosphorylation for Hijacking of Cullin-RING Ligases. mBio, 8(4), e01213-17.

Publication 2017

TOP10 Escherichia coli cells

Cells Endotoxin Escherichia coli Nsp1 Plasmid Transient Vectors

Corresponding Organization :

Other organizations : Virginia–Maryland College of Veterinary Medicine, University of Maryland, College Park, National Institute of Allergy and Infectious Diseases, National Institutes of Health

Top 5 similar protocols

Variable analysis

independent variables

Wild-type and mutant forms of NSP1
FLAG-tagged β-TrCP
DN-hCUL3-FLAG

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive control: pcDNA3-DN-hCUL3-FLAG (gift from Wade Harper, Addgene plasmid 15820)
Negative control: not mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!