Comprehensive Metabolic and Inflammatory Profiling

For analysis of lipids, glucose, uric acid, creatinine and the inflammation marker high sensitivity C-reactive protein (hs-CRP), blood samples were drawn in the morning between 8:00 and 10:00 am after a period of overnight fasting. Serum levels of total cholesterol and high-density lipoprotein (HDL) cholesterol were measured with an enzymatic colorimetric method, low-density lipoprotein (LDL) cholesterol with an enzymatic method and total triglycerides with a colorimetric UV method, all on a Roche Modular P chemistry analyzer (Roche, Basel, Switzerland). Fasting blood glucose was measured using a hexokinase method. HbA1c was determined in whole blood (EDTA-anticoagulated) by means of turbid metric inhibition immunoassay on a Cobas Integra 800 CTS analyzer (Roche Diagnostics Netherland BV, Almere, The Netherlands). Insulin resistance was measured by calculating the ratio total triglycerides/HDL cholesterol [27 (link)]. Serum uric acid and creatinine were measured on a Roche Modular P chemistry analyzer (Roche, Basel, Switzerland). The hs-CRP was determined by nephelometry (BN II system Siemens, Marburg, Germany).