Multiplex qRT-PCR for Swine Enteric Pathogens

To detect swine enteric pathogens, real-time reverse transcription polymerase chain reaction (qRT–PCR) was performed for 10 viral pathogens [PDCoV, porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), porcine enteric alphacoronavirus (PEAV), swine acute diarrhea syndrome coronavirus (SADS-CoV), porcine torovirus (PToV), porcine sapelovirus (PSV), group A rotavirus (RVA), group B rotavirus (RVB), group C rotavirus (RVC)] using a commercial one-step qRT–PCR kit (THUNDERBIRD™ Probe One-step qRT–PCR kit, TOYOBO, Osaka, Japan). The previously reported 10 qRT-PCR assays were performed using the target gene-specific primer and probe sets of each viral pathogen, as described in their studies [7 (link),25 (link),26 (link),27 (link),28 (link),29 (link),30 (link)]. The reaction composition and conditions were performed according to the manufacturer’s instructions.

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Kim H.R., Park J., Lee K.K., Jeoung H.Y., Lyoo Y.S., Park S.C, & Park C.K. (2023). Genetic Characterization and Evolution of Porcine Deltacoronavirus Isolated in the Republic of Korea in 2022. Pathogens, 12(5), 686.

Publication 2023

THUNDERBIRD™ Probe One-step qRT–PCR kit

Alphacoronavirus Assays Gene Group b rotavirus Group c rotavirus Pathogen Porcine Porcine epidemic diarrhea virus Porcine sapelovirus Porcine torovirus Primer Real time polymerase chain reaction Reverse transcription Rotavirus Sads cov Transmissible gastroenteritis virus

Corresponding Organization : Kyungpook National University

Other organizations : Animal and Plant Quarantine Agency, Konkuk University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Detection of 10 viral pathogens: PDCoV, PEDV, TGEV, PEAV, SADS-CoV, PToV, PSV, RVA, RVB, RVC

control variables

None explicitly mentioned

controls

No positive or negative controls were explicitly mentioned in the input.

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