Following reagents were used at different stages of sample processing and DNA isolation: EDTA (0.5 M, pH 8.0), NaCl (5 M), PVPP (Mol wt 40,000), Guanidine thiocyanate (4 M), Sodium-acetate (3 M, pH 5.2), Potassium acetate (5 M, pH 5.2), N-Laurylsarcosine (10%), Glass beads (2.5 mm), Zirconia beads (0.1 mm), Ethanol (96%), Hydrochloric acid (HCl), Sterile deionized water (H2O). All the chemicals used in this study were purchased from Sigma-Aldrich, USA.
Bag S., Saha B., Mehta O., Anbumani D., Kumar N., Dayal M., Pant A., Kumar P., Saxena S., Allin K.H., Hansen T., Arumugam M., Vestergaard H., Pedersen O., Pereira V., Abraham P., Tripathi R., Wadhwa N., Bhatnagar S., Prakash V.G., Radha V., Anjana R.M., Mohan V., Takeda K., Kurakawa T., Nair G.B, & Das B. (2016). An Improved Method for High Quality Metagenomics DNA Extraction from Human and Environmental Samples. Scientific Reports, 6, 26775.
Other organizations :
Translational Health Science and Technology Institute, Novo Nordisk Foundation, University of Copenhagen, P. D. Hinduja Hospital and Medical Research Centre, Maulana Azad Medical College, Madras Diabetes Research Foundation, Osaka University
No positive or negative controls were explicitly mentioned
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