Visualizing ROS Distribution in Plant Leaves

The hydrogen peroxide (H₂O₂) and peroxide anion (O

_{2}^{- .}

) were detected following the manufactural instructions of a hydrogen assay kit (A064-1-1, Jiancheng, Nanjing, China) and method described by Xu et al. (2015 (link)). To further explore the ROS distribution in plant leaves, the visualization of H₂O₂ and O

_{2}^{-}

was conducted using 3,3'-diaminobenzidine (DAB) and nitroblue tetrazolium (NBT) staining according to the methods as described previously (He et al., 2019 (link)).

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Tian F., Han C., Chen X., Wu X., Mi J., Wan X., Liu Q., He F., Chen L., Yang H., Zhong Y., Qian Z, & Zhang F. (2022). PscCYP716A1-Mediated Brassinolide Biosynthesis Increases Cadmium Tolerance and Enrichment in Poplar. Frontiers in Plant Science, 13, 919682.

Publication 2022

A064-1-1

Anion Assay H2o2 Hydrogen Nitroblue tetrazolium Peroxide Plant leaves Staining methods

Corresponding Organization : Sichuan Agricultural University

Other organizations : State Forestry and Grassland Administration

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Variable analysis

independent variables

Hydrogen peroxide (H2O2) concentration
Peroxide anion (O2-) concentration

dependent variables

Hydrogen peroxide (H2O2) detection
Peroxide anion (O2-) detection
Visualization of H2O2 and O2- distribution in plant leaves

control variables

Adherence to the manufactural instructions of the hydrogen assay kit (A064-1-1, Jiancheng, Nanjing, China)
Adherence to the method described by Xu et al. (2015)
Adherence to the methods described by He et al. (2019) for H2O2 and O2- visualization

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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