Micropeptide Induces Cancer Cell Death

Example 9

The ORF encoding the micropeptide of SEQ ID NO: 3 was cloned in frame with the HA epitope tag in the pMSCV retroviral vector. Western blot and qPCR analysis demonstrated that the micropeptide of SEQ ID NO: 3 was successfully expressed after retroviral transduction, and that the protein product was stable (FIG. 23A y 23B).

Importantly, overexpression of the micropeptide of SEQ ID NO: 3 induces massive cell death in cancer cell lines (A549, human lung cancer and HCT116, human colorectal cancer) (FIG. 23C).

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US11858972B2. Micropeptides and uses thereof (2024-01-02). FUNDACIÓ PRIVADA INSTITUT D'INVESTIGACIÓ ONCOLÒGICA DE VALL HEBRON [ES]. Inventors: Maria Abad Méndez [ES], Olga Boix Sánchez [ES], Emanuela Greco [ES], Iñaki Merino Valverde [ES].

Patent 2024

Cancer Cell death Colorectal cancer Epitope Frame Human Lung cancer Protein Retroviral Vector Western blot

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Variable analysis

independent variables

Overexpression of the micropeptide of SEQ ID NO: 3

dependent variables

Cell death in cancer cell lines (A549, human lung cancer and HCT116, human colorectal cancer)

control variables

The ORF encoding the micropeptide of SEQ ID NO: 3 was cloned in frame with the HA epitope tag in the pMSCV retroviral vector

controls

Western blot and qPCR analysis demonstrated that the micropeptide of SEQ ID NO: 3 was successfully expressed after retroviral transduction, and that the protein product was stable (FIG. 23A and 23B)

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