Quantification of Adipose-Derived Stem Cells

The total cell numbers were determined using a NucleoCounter NC-100 (Chemometec, Allerød, Denmark). SVF was analyzed by FCM to determine the ratio of ASCs to SVF. Cells in SVF were incubated with FITC-conjugated CD31 (BD Biosciences, New Jersey, USA) and PE-conjugated CD34 (BD Biosciences). The labeled cells were washed with phosphate-buffered saline and analyzed using a FACSCalibur flow cytometer (CellQuest software, BD Biosciences). ASCs were defined as the CD31⁻/CD34⁺ subpopulation [11 (link)-13 (link)]. ASCs in SVF were calculated as follows: ASCs in SVF (cells) = total cell number × ASC:SVF.

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Nishimura A., Kumagai T., Nakatani M, & Yoshimura K. (2016). Method for selective quantification of adipose-derived stromal/stem cells in tissue. Journal of Biological Methods, 3(4), e58.

Publication 2016

NucleoCounter NC-100 FITC-conjugated CD31 PE-conjugated CD34 FACSCalibur flow cytometer

Cells Fitc Phosphate Saline Subpopulation

Corresponding Organization :

Other organizations : Kaneka (United States), Jichi Medical University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Total cell number
Ratio of ASCs to SVF
ASCs in SVF (cells)

control variables

None explicitly mentioned

controls

Positive control: None mentioned
Negative control: None mentioned

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