Evaluating Scaffold Osseointegration by SEM and EDX

Two unstained thick sections (50–80 µm) per material and time group were examined using energy dispersive X-ray spectroscopy (EDX) and scanning electron microscopy (SEM) and prepared analogously to Section 2.1.5. The scaffolds were assessed in the SEM regarding osseointegration based on the surface structure of the thick sections and the contact surface to the surrounding bone. EDX was used to determine the presence of material particles based on the presence of magnesium (Mg) ions or an increased concentration of calcium (Ca) and phosphate (P) ions compared to the surrounding bone.

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Hemmerlein E., Vorndran E., Schmitt A.M., Feichtner F., Waselau A.C, & Meyer-Lindenberg A. (2024). In Vivo Investigation of 3D-Printed Calcium Magnesium Phosphate Wedges in Partial Load Defects. Materials, 17(9), 2136.

Publication 2024

Corresponding Organization : Ludwig-Maximilians-Universität München

Other organizations : University of Würzburg

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Variable analysis

independent variables

Material type
Time group

dependent variables

Presence of material particles based on the presence of magnesium (Mg) ions or an increased concentration of calcium (Ca) and phosphate (P) ions compared to the surrounding bone
Osseointegration based on the surface structure of the thick sections and the contact surface to the surrounding bone

control variables

Thickness of the sections (50–80 µm)
Preparation method (analogous to Section 2.1.5)

controls

Positive control: Not specified
Negative control: Not specified

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