Quantification of Influenza Virus Nucleoprotein

Lung tissue lysates from the mice were prepared from tissues collected at 3 dpi. Lysates of sh-NMBR cells, sh-Luciferase cells, PR8-infected sh-NMBR cells, and non-infected controls were prepared from samples harvested at 0, 6, and 12 hpi. All lysates were resolved by SDS-PAGE in 10% polyacrylamide gels. Bands were detected using rabbit anti-H1N1-NP (generated in our laboratory) as previously described [27 (link)] and anti-hNMBR (ab134141, Abcam, USA). β-Actin was detected using a rabbit anti-actin polyclonal antibody (R019, TransGen Biotech, China). The secondary antibodies for detection were goat anti-mouse (125229, Jackson ImmunoResearch Laboratories, USA) and goat anti-rabbit antibodies (131879, Jackson ImmunoResearch Laboratories). The blots were developed using the FluorChem M Imaging System (ProteinSimple, USA).