Tissue processing and quantitative PCR (qPCR) were performed as previously described 30 (link). Sigma-Aldrich custom primers were used for genes of interest with the sequences shown in Supplementary Table 1 . All qPCR gene expression was normalized to the housekeeping gene GAPDH.
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Tissue and qPCR Analysis Protocol
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Other organizations : The Ohio State University Wexner Medical Center, The Ohio State University
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Variable analysis
independent variables
- Tissue processing and quantitative PCR (qPCR) methods
- Gene expression
- GAPDH (housekeeping gene) for normalization of qPCR gene expression
- No positive or negative controls were explicitly mentioned.
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