To prepare this formulation, activation of the carboxylic
acid groups of stearic acid was needed. It was achieved as described
in our previous study.39 (link) We dissolved 284
mg of stearic acid (MW: 284.48, Acros Organics, Geel, Belgium), 206
mg of 1-(3-(dimethylamino)propyl)-3-ethylcarbodiimide hydrochloride
(EDC; Acros Organics, Geel, Belgium), 140 mg of N-hydroxysuccinimide (NHS; Acros Organics, Geel, Belgium), and 0.250
mL of triethanol amine (TEA; Molekula GmbH, Munich, Germany) in 20
mL of DMSO in a beaker. We stirred the mixture for 2 h at 80 °C
and another 24 h at RT. In addition, we slowly added the activated
solution containing stearic acid to the CSFI solution, stirred for
10 h at 60 °C, and kept it at RT for another 24 h. The resulting
solution (CSFISA) was stored at −20 °C until further use.
acid groups of stearic acid was needed. It was achieved as described
in our previous study.39 (link) We dissolved 284
mg of stearic acid (MW: 284.48, Acros Organics, Geel, Belgium), 206
mg of 1-(3-(dimethylamino)propyl)-3-ethylcarbodiimide hydrochloride
(EDC; Acros Organics, Geel, Belgium), 140 mg of N-hydroxysuccinimide (NHS; Acros Organics, Geel, Belgium), and 0.250
mL of triethanol amine (TEA; Molekula GmbH, Munich, Germany) in 20
mL of DMSO in a beaker. We stirred the mixture for 2 h at 80 °C
and another 24 h at RT. In addition, we slowly added the activated
solution containing stearic acid to the CSFI solution, stirred for
10 h at 60 °C, and kept it at RT for another 24 h. The resulting
solution (CSFISA) was stored at −20 °C until further use.
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