For the sorting of co-cultured HaCa4 and LEC, and PDV and LECs, cells were stained as previously described [96 (link)] using the primary antibody CD31 (Rat MEC 13.3,1:100, B.D. Franklin Lakes, NJ, USA) and the secondary antibody anti-rat AlexaFluor 647 (1:200, Jackson ImmunoResearch, West Grove, PA, USA). HaCa4 or PDV cells were stained with CFSE Cell Proliferation Kit (ThermoFisher Scientific, Waltham, MA, USA) following the manufacturer’s instructions, and CD31+ LECs were sorted with an Aria II FACS sorter (B.D. Franklin Lakes, NJ, USA) excluding dead cells and doublets.
Free full text: Click here
