To obtain sufficient number of ILC2 for CHIP, an immortalized ILC2 line was established with small intestinal laminal propria (SiLP) ILC2 from C57BL/6 mice by spontaneous mutation and selection (Supplemental Fig. 1 ). We named this cell line ILC2/b6 line. ILC2/b6 cells were maintained with 10ng/ml of IL-2, IL-7 and IL-33. For CHIP analysis, we grew ILC2/b6 cells on OP9-Dll1 stroma. Around 107 ILC2/b6 cells were re-purified by Magnet-activated cell sorting with CD45 beads (Miltenyi Biotech). CHIP was performed as we described (16 (link)). Primers to detect the CSL binding site at the Rorc locus were previously described (17 (link)).
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Establishing Immortalized ILC2 Cell Line for ChIP
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Corresponding Organization : Albany Medical Center Hospital
Other organizations : Second Affiliated Hospital of Zhejiang University, University of California, Davis, National Institute of Allergy and Infectious Diseases, National Institutes of Health, National Cancer Institute, Center for Cancer Research, University of Michigan–Ann Arbor
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- Establishment of an immortalized ILC2 line (ILC2/b6) from SiLP ILC2 of C57BL/6 mice by spontaneous mutation and selection
- Characteristics and behavior of the ILC2/b6 cell line
- Maintenance of ILC2/b6 cells with 10ng/ml of IL-2, IL-7 and IL-33
- Growth of ILC2/b6 cells on OP9-Dll1 stroma for CHIP analysis
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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